LIFE 212 Lecture Notes - Enzyme Kinetics, Tyrosinase, Transmittance

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Spectrophotometry: employs beer"s law: relates absorbance to concentration of substances in solution. Extraction of enzyme tyrosinase using high salt precipitation. Extinction coefficient (e) : also called molar absorptivity. Absorbance of a unit concentration of a solute in a solution, depends on the chemical nature of the substance, has the units of reciprocal concentration and length (m-1 cm-1) Direct linear relationship of absorbance to concentration of a solute. The equation follows the equation of a straight line, y = mx + b. M is the slope of the line, b is the y intercept. Determining the concentration of a substance in a solution where e is unknown or when a substance does not obey beer"s law. Determining the extinction coefficient of a substance by determining the slope. They speed up the rate of a chemical reaction without being used up. Targets are limited by the molecular structure of the enzyme"s active site. The study of the rate of enzyme-catalyzed chemical reactions.

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