LIFE 212 Lecture Notes - Enzyme Kinetics, Biotin, Chemical Substance
30 Mar 2014
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Document Summary
Is the most often used theory and technique in a biochemistry lab. Is a technique that measures the interaction of electromagnetic radiation with molecules, atoms, or ions. Each substance absorbs a unique wavelength of light (monochromatic light) from the light spectrum. Substances appear a certain color because they absorb certain wavelengths of light and transmit others. Lmax: wavelength at which absorbance is the greatest. Lmax is characteristic of each chemical substance and provides information on the electronic structure of the analyte. Is the rate of a physiological activity plotted against wavelength of light. Direct linear relationship of absorbance (a) to concentration (c) of a solute. A = absorbance, e = extinction coefficient, c = concentration. The equation follows the equation of a straight line. Where m is the slope of the line and b is the y intercept. Determining the concentration (c) of a substance in solution when e is unknown or when a substance does not obey beer"s law.
