MBB 222 Study Guide - Rede Ferroviária Nacional, Nucleosome, Intron

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Total marks for problem set = 41 marks. Problem set is due on friday, november 29th, 2013 in class. Hand in your problem set to your teaching assistant for marking in class. Answers written in pencil will not be accepted. Zero marks will be given to questions with answers that are not legible! This problem set is worth 2% towards your final mark in the course. Disrupts hydrogen bonds between complementary bases, yielding single-stranded dna molecules. Allow primers to complementary base pair at specified site on the single-stranded dna template. Answer: (any of the three are fine 1 mark each) The fluorescently labeled dna fragments will move across a path of a laser which allow each color dye to fluoresce at different wavelengths (and therefore correctly determine the position of each base) Automated dna sequencing equipment can eliminate the need for radioactive isotopes to label.

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