HMB200H1 Lecture Notes - Lecture 20: Bromodeoxyuridine, Dentate Gyrus, Netrin
Document Summary
Lecture 20 : brain development. Folding over = gastrolation establishes position of cells. This cell mass has no special shape. This ball of cells will fold over later on = gastrulation. This folding over creates a line (longitudinal axis) Formation of neural tube (18- 24 days). Most cells are on the inside they are embryonic stem cells. Embryonic stem cells are undifferentiated differentiated later on. Take these cells out using pipette, and put onto petri dish to be grown. These cells can be altered and mutated (dna can be changed) Put into test tube and add extra dna = transgenic cell. These transgenic cells can now duplicate. If sperm represents mutant cell, its babies will have mutant skin colour (all be black) After birth = p (postnatal) knockout mice: don"t have the gene get the gene introduced to replace the original dna. Mutant dna missing an essential piece (the knockout) and this mutant has to replace the wildtype dna.
