CHEM 313 Chapter Notes -Shikimic Acid, Galactose, Reagent
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Chem 313 problem set #5, 2010 answers: iptg is a common chemical used for the induction of the lac-operon in many recombinant gene technologies. How is iptg prepared from galactose: outline the steps you would take to prepare each of the four following disaccharides (starting from any appropriate unprotected carbohydrate precursor), x-gal is a commonly used reagent in bacterial cloning technologies. It produces a convenient blue product (after oxidation within the cell, shown on the right) to easily identify a link between phenotype and genotype. Cells that do not express - galactosidase do not produce this blue colour and appear white. How would you prepare x-gal from glucose and 5-bromo-4-chloro-3-hydroxy indole: the product on the right below is an important precursor in the shikimate pathway, which produces shikimic acid. It leads to an intermediate that is useful in the biosynthesis of a variety of metabolites. This precursor has been chemically prepared from the spontaneous rearrangement shown below (j.
