BIOCH200 Lecture Notes - Nucleic Acid Thermodynamics, Gc-Content, Dna Replication
10 Apr 2014
School
Department
Course
Professor
Document Summary
Bioch 200 nucleotides and nucleic acids con"t (january. *tm = midpoint of melting (halfway between denaturing and annealing: *varies based on base composition. *the higher the gc content, the stronger the base stacking forces, the more stable the dna structure, and therefore the higher the temperature is needed for denaturing (higher tm) Because there are 3 hbonds that occur between guanine and cytosine and only 2 hbonds that occur between adenine and thymine. Renaturing reformation of dsdna so that it regains its native conformation. Steps bases paired, nucleation occurs, then zippering happens: ph and salt concentration also affect tm, *ph will affect tm because it affects the protonation state of dna. Can have negative consequences and lower tm: salt concentrations: *because the positive ions help shield the negative charges on the phosphate groups. This helps stabilize the structure of dsdna: ex.
