Chromosome Mapping (determining the relative positions of genes on a chromosome) - This is a two point cross since it involves two loci on a chromosome.
Follow the directions for the crosses below involving 2 traits located together on the same chromosome (Drosophila Chromosome IV): eyeless and shaven bristles. Please only typed answer, no hand-written work or screen shots please.
1. a. Perform a monohybrid cross between a female fly with the eyeless mutation and a male fly with shaven bristles. Show results for the F1 offspring. (NOTE: You will use an F1 female in the next cross.)
ENTER DATA HERE:
Question: Are the phenotypes of the F1 offspring what you would have predicted for this cross: why or why not?
Question: After looking at your F1 offspring, which allele is dominant: wild-type eyes or eyeless? How can you tell?
Question: After looking at your F1 offspring, which allele is dominant: wild-type bristles or shaven bristles? How can you tell?
Draw the Chromosome IV pair of an F1 offspring: (HINT: One chromosome was inherited from the mother, with the eyeless allele at the eye shape location (locus) and the wild-type allele at the bristles locus. The second chromosome was inherited from the father, with the wild-type allele at the eye shape locus and the shaven bristle allele at the bristles locus.)
1. b. Perform a testcross between an F1 female offspring from the previous cross and a new male with both the eyeless and the shaven bristle traits to produce an F2 generation.
ENTER DATA HERE:
Question: What phenotypes are found in the F2 offspring?
Question: Are the phenotypes of the F2 offspring what you would have predicted for this cross: why or why not?
Draw the 2 different Chromosome IV pairings you expected in an F2 offspring: (HINT: The F1 female will donate one or the other of the chromosomes you drew above to each offspring. The new male with both the eyeless and the shaven bristle traits can only donate one type of chromosome, with both mutations.)
Question: What are the 2 phenotypes that would result from the 2 different chromosome pairings you just drew?
Question: What are the other 2 phenotypes that you obtained in your F2 offspring? What process, which occurred during meiosis, was responsible for the creation of these recombinant phenotypes?
Draw a map that shows the map distance (in map units or centimorgans) between the locus for the shaven bristle allele and the locus for the eyeless allele. (NOTE: You need to add the proportion of SV;EY flies to the proportion of wild flies OR add the number of SV;EY flies and wild flies and then divide by the total number of flies. Then multiply either number times 100.)âSHOW ALL CALCULATIONS BELOW
CALCULATIONS:
CHROMOSOME MAP:
There are several different ways to draw maps of the chromosomes. You can draw a simple line graph using a free online graphing tool, such as:
http://www.onlinecharttool.com/
http://nces.ed.gov/nceskids/createagraph/
Or you can simply use your keyboard to create a line with the dash or underline, with the abbreviations for the genes inserted, and then put the map units below, such as:
___________A_____________________B_________
50 map units
