BIOL 2020 Lecture Notes - Secretion, Ribosomal Rna, Secretory Protein
12 Apr 2014
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Document Summary
When we mash up a cell- majority of microsomes come from the er. Major amount of membrane in a cell in the er (jammed packed with membrane) Rough (rer): ribosomes bound to cytosolic surface of er. Protein reticulon (group of them) when they isolate the er (by mashing the cell) these critters (organelles) form vesicles called microsomes. If you isolate the smooth ones you can ring a lot of this protein (reticulon) Ribosomes inside the cisternae are called free ribosomes. Continuous with rer (see slide 7 - cell 4 er) The inside of the rough er is called the lumen (also referred to the inside of organelles) Scanning electron microscope: coat the 3d shape with electron dense metals and then we bombard it with electrons and measure the elections that bounce off. Phospholipids and sphingolipids are synthesized in the p face and then flippase flips them to the e face.
