PSYB45H3 Lecture Notes - Lecture 4: Clathrin, Lysosome, Mannose
Document Summary
Clathrin binds to m-6-p receptor on the cytosolic face: the vesicle then pinches off and fuses to late endosomes which mature into lysosomes, the receptors then go back into the trans golgi (recycled) Lysosomal enzyme is made in the er, once it enters the golgi the sugar is phosphorylated. Then it goes to trans golgi and associates with receptors. On the cytosol side clathrin helps form vesicles and then it fuses with late endosomes. Slide 8: there were people who had lysosomes filled with undigested material. The problem was that enzymes weren"t getting directed properly and getting secreted: this is called i-cell disease. They purified secreted lysosome enzymes and compared it with normal patients. Slide 9: enzymes hydrolyze macromolecules, if it"s not acidic enough they won"t work, all cells have them but immune cells have a lot more. Lysosomes have many proton pumps that drive hydrogen into the lumen of the lysosome.
