PSYC 4034 Study Guide - Photographic Emulsion, Osmosis, Functional Magnetic Resonance Imaging
Document Summary
Histological methods [fix, slice, stain, examine: you have to make sure that you have injected what you wanted to inject in the right place. You must remove the blood from the brain in the animal. The vascular system must be put through the vascular system. It makes the brain slightly harder than in the brain. You always want to observe the lesion to verify the precise location of the damage: prefuse must be flushed out. Put needle in the heart and remove blood and replace with saline: formalin normal, fixate physically able to be handled. It also prevents any tissue breakdown and autolysis. any tissue at an adjustable pre-determined thickness. In order to cut it, it has to made as hard as possible. There are 2 ways to do this: it can be dropped in wax and left until it hardens and then sliced or a freezing microtome can be used. It freezes the brain. used to enhance the contrast.
