MIP 300 Lecture Notes - Plasmid, Bacteriocin, Deoxyribonuclease

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lemondog142

25 Jun 2014

School

CSU

Department

Microbio, Immun, Pathology

Course

MIP 300

Professor

Erica Suchman

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Document Summary

Prokaryotes may pick up exogenous dna in 3 ways. No integration into host cell dna, independent replication, plasmids. Replicons: contain origins of replication independently replicating, stably inherited. Episomes: may exist as plasmids or integrate into host dna (some) Conjugative plasmids: carry genes for conjugation pili and sex pili production (some) Resistance (r) factors: carry genes that confer resistance to antibiotics, often conjugative. Col plasmids: contain genes to produce bacteriocins kill other bacteria. Virulence plasmids: contain genes to make bacteria more virulent or pathogenic, carry genes for. Metabolic plasmids: contain genes to allow bacteria to metabolize new substances, e. g. pesticides. R = rough microscopic appearance, nonpathogenic, no capsule. Inject mouse with strain r mouse lives. Inject mouse with strain s mouse dies. Inject mouse with boiled strain s mouse lives. Inject mouse with boiled strain s and non-boiled strain r mouse dies. Take heat killed strain s, treat with protease (digests proteins), mix with strain r, inject mice mice die.

Related Questions

Some viruses have a lysogenic lifecycle. What are these viruses called in bacteria when they integrate into the bacterial chromosome? What are they called if the host cell is eukaryotic (you may have to look that one up)? What is an âattachment siteâ? If the virus enters the lytic cycle, which two enzymes are involved in the excision of the DNA from the host cell chromosome? Which of them was also involved in the insertion?

jaderabbit973

1
Which of the following is a component of the HIV virus ?
A
DNA
B
Capsid
C
Nucleus
D
CD4 receptor
Answered
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2
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Homework
2
What is the function of Reverse Transcriptase?
A
To insert the viral RNA into the host DNA.
B
To make an RNA copy of the viral RNA.
C
To make an RNA copy of the viral DNA.
D
To make a DNA copy of the viral RNA.
E
To insert the viral DNA into the host DNA.
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3
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3
Which of the following stages describes a HIV patient in who has a large number of severe opportunistic infections?
A
Primary HIV infection-lasts for a few weeks
B
Clinically asymptomatic stage-lasts for an average of ten years
C
Symptomatic HIV infection
D
Clinical AIDS
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4
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4
Which of the following individuals should be tested for HIV?
A
A newborn child
B
Alex, who studies excessively
C
Sarah, who takes birth control
D
Joe, who uses intravenous steroids
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5
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5
Harry engaged in risky activity that may have lead to exposure to HIV. Two and a half weeks later, he had an HIV test done and it showed a positive result. Which test most likely was done?
A
CD4 test
B
HIV antibody test
C
PCR test
D
Viral load test
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6
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6
How does an Integrase inhibitor work against the HIV virus?
A
It inhibits the generation of viral DNA from viral RNA.
B
It prevents the entry of HIV into the host cell.
C
It inhibits viral proteins from being inserted into new virus particles.
D
It prevents viral DNA from being inserted into host DNA.
E
It prevents the generation of new viral proteins.
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7
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7
How does a Protease inhibitor work to treat HIV infection?
A
It prevents the release of new viral particles.
B
It cleaves viral proteins interfering with their function.
C
It prevents the cleavage of host DNA preventing viral DNA from being inserted into host DNA.
D
It cleaves Reverse transcriptase preventing the generation of viral DNA.
E
It prevents the binding of gp120 to the CD4 protein.
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8
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8
Which of the following describes a combination therapy for HIV infected persons?
A
A preparation used for stimulating a viral response in the body.
B
A single drug given to HIV infected people since interactions with other medications may promote complications in treatment.
C
A drug cocktail-or-combination of antiviral drugs given to HIV infected people since they can be more effective than when given individually
D
A preparation used for stimulating an immune response in the body.
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9
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9
answerable question reference
For HIV to reproduce, which of the following is most critical?
A
Infecting new subjects
B
Producing a Provirus
C
Entering the host cells
D
Taking over the function of DNA in the cell
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10
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10
HIV is a virus which has the following characteristic?
A
It takes of the RNA of the host cell which subsequently by way of transcription and translation makes new virus
B
It attacks the RNA of the host cell causing cellular death
C
It attacks the DNA of the host cell causing cellular death
D
It takes over the DNA of the host cell which subsequently by way of transcription and translation makes new virus

1. List some of the proteins that are involved in homologous recombination

2. Some viruses have a lysogenic lifecycle. What are these viruses called in bacteria when they integrate into the bacterial chromosome? What are they called if the host cell is eukaryotic (you may have to look that one up)? What is an âattachment siteâ? If the virus enters the lytic cycle, which two enzymes are involved in the excision of the DNA from the host cell chromosome? Which of them was also involved in the insertion?

3. What are two examples of site-specific recombination

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MIP 300 Lecture Notes - Plasmid, Bacteriocin, Deoxyribonuclease

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