BIOL 2051 Lecture Notes - Phospholipid, Carboxysome, Gram-Positive Bacteria
Document Summary
Structure & function: name 6 abilities of living cells. Brightfield: simplest, specimen illuminated, magnified 1000x, specimen stained to increase contrast. 10x objective = 100x total magnification 100x objective = 1000x total magnification. Cell has black background, light shone directly on specimen: visualize live samples, no staining required. Shadow cast, dimension of cells visible: visualize live samples, no staining required. Visualization of auto fluorescent molecules or fluorescent stains. Required uv lamp: cells usually die or become damaged because of the uv light. Dapi: used to stain, typically stains the nucleus. Must cut the cell, and stain them as well. Describe what that value means in practical terms when viewing a specimen. 2 m - this means any two organisms next to each other wont be seen as next to each other. 2 m 0. 2microns is the size of the smallest free-living pathogenic bacterium. Good for nutrient and waste exchange: discuss the problems a cell might have it were less than 0. 2microns.
