Biochemistry 2280A Study Guide - Tryptophan, Sticky And Blunt Ends, The Techniques

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Cell walls are always found in prokaryotes and sometimes in eukaryotes. Information is stored in dna in the form of genes. The human genome encodes ~25,000 different protein encoding genes. In any one cell type ~10,000 are expressed at various levels. The expression of the correct genes is essential for growth and differentiation of all cells. Most disease states result from alterations in expression of one or more genes. By manipulating gene expression, we have the potential to prevent or reverse disease. Gene manipulation can be done at transcription and/or translation (fig 7-1) The rna step allows genes to be expressed at different levels. (fig 7-2) Rna allows expression of a gene can be stopped quickly because it can be degraded. Rna provides additional opportunities to regulate the expression of genes. Points at which gene expression can be regulated in a eukaryotic cell.