BIO 1140 Study Guide - Red Blood Cell, Osmosis, Sucrose

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14 Jul 2014
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Time of hemolysis of sheep red blood cells in penetrating substances. Water asolutions classified using pubchem substance bhemolysis appeared to be instantaneous chemolysis was not attained in 1200 seconds. <2b section average measurements (s) for section measurements (s) There are a number of factors that affect the diffusion of solutes into sheep erythrocytes. Most importantly, all solutions are made of penetrating substances. This is essential to the lab: because the solutes can pass through the cell membrane, they are able to create hyposmotic environment. In this environment, osmosis causes the red blood cells to take in water and eventually causes hemolysis. The amount of time for hemolysis is ultimately influenced by the permeability of the erythrocyte to the solute. Factors of the solute which affect permeability are: size of the molecule, the polarity of solute, temperature of the solution, and ph of the solution. The larger the molecule, the more difficult it is to diffuse into the erythrocyte.

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