HSS 2305 : HSS2305_Chapter18_Oct28.docx

• School: University of Ottawa
• Department: Health Sciences
• Course: HSS 2305
• Professor: All

Document Summary

Chapter 18 techniques in cell and molecular biology. Condenser lens gathers diffused rays from light source and illuminates the specimien. Objective lens collects the light rays focused on the specimen by the condenser lens. Ocular lens uses the image formed by the objective lens to make an enlarged and virtual image. 18. 2: factors that allow an object to actually be observed. Bright-field microscope uses a bright background against which the image of the specimen must be contrasted. Preparation of specimens for bf microscopes: whole mount = an intact object (living or dead, section = a thin slice of tissue. a phase/spot. Used in fret (fluorescence resonance energy transfer) to measure distances btw fluorophores. Ex. measure changes in distance btw two parts of a protein: dsred = distantly related red fluorescent tetrameric protein. Laser scanning confocal microscopy: marvin minsky: produces an image of a thin plane situated within a much thicker specimen.