BIOL308 Lecture Notes - Molecular Cloning, Oligonucleotide, Laparotomy

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Start from protein (which is isolated) and working back to the information contained in dna. Start with genetic information and work towards the product. Protein is general product, however it can sometimes be rna as the product. Two links between both types, in other words tools that assist with both methods: Note: both approaches will give you the exact same information, it all depends on what you have to start with. Isolate protein on the basis of its molecular function. Because there are multiple codes it can be hard. Create oligonucleotides which correspond to certain parts of the amino acid sequence. Using the oligonucleotides as probes to select cdna or genomic clone encoding the protein from this library. Using cdna to find the real gene in the organism. Isolate genomic clone that responds to a specific trait, this trait is one that is altered trait in mutants.

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