BIOL308 Lecture Notes - Lecture 5: Shotgun Sequencing, Satellite Dna, Purine
16 Jul 2014
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Document Summary
Reassociation is inversely proportional to the genome (dna) size(refer to above photo) Mouse satellite - repetitive genes in the mouse genome. The dna sources are: a synthetic dna duplex of poly a and poly u polynucleotide chains; mouse satellite dna, a fraction of mouse dnain which the same sequence is repeated many thousands of times; Ms2 dna (bacteriophage) t4 dna(more complex bacteriophage then ms-2) E. coli dna; calf dna-nonrepetitive fraction (highly repetitive dna fraction was removed) Unique, protein coding sequences could be "purified" from the mixture. Faster then shotgun sequencing (repetitive sequences are not sequenced) C-paradox; no correlation between amount of dna in genome and apparent complexity. No correlation between the amount of dna [size of genome] and the apparent complexity of organisms. More dna present than we can account for . Proportions of different sequence types vary among eukaryotic genomes. Analyze trends in the red bars; proportions don"t stay the same.
