BCH311H1 Lecture Notes - Rna Polymerase I, Radioactive Tracer, Primary Transcript

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22 Jul 2014
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Only has 1 rnap to bind promoter for initiation. Primary transcript of mrna modification has little to no modification. trna and rrna transcripts undergo modification. Primary rna transcript serves as mrna and is elongated. Activation/repression done by regulatory proteins e. g. lac operon. Primary rna transcripts are extensively modified e. g. splicing, capping. Simultaneous transcription and translation so no spatial or temporal differences. Transcripts are exported out of nucleus into cell cytoplasm. Not need tfs for transcription, only to recognise the promoter sequence. Termination by rnap at the end of the gene. Similarities: (1) overall rnap quite similar between prok and euk. Rna synthesised de novo as opposed to dna. (2) rna synthesis is in 3 stages: initiation, elongation and termination i. e. nascent rna 5" 3" synthesis from n-terminus c-terminus as it is complementary to the dna template. Footprinting: determines where specific dbp (dna binding protein) latches on to dna strand. i.