BIOB12H3 Chapter Notes -Lac Repressor, Regulatory Sequence, Operon
Document Summary
Week 7a: induction of the lac operon in wild type and mutant strains of e. coli. Week 7b: lac operon: interpretation of week 7a data and design experiment to test regulation of lac operon. Week 8b: data analysis of group experiment and restriction mapping assingment. Recognize the type of mutation present based on the induction of beta- galactosidase production. To understand the use of colourimetric substrates in enzyme assays. To understand the use of enzyme activity as a marker for gene. To understand the mechanism of control underlying induction of the lac expression operon. Transcription involves trans-acting factors (e. g. rna polymerase and other factors) moving to and interacting with specific dna regulatory sequences (cis-acting sequences) to alter gene expression. Dna binding proteins (transcription factors) that coordinate transcription of specific genes or sets of genes needed by the cell or organism to respond to stimuli. Usually, the regulatory dna sequences lie upstream" from the gene in the region called the promoter.
