BIOL130L Study Guide - Final Guide: Sodium Hydroxide, Enol, Root Cap
13 Sep 2014
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Place yeast, water and sand into a mortar and grind. The yeast cells are held together by a starchy binding material. Glucan (a cellulose-like polysaccharide) is in the cell walls and glycogen, proteins and nucleic acids in the cytoplasm. Grinding with sand ruptures cell wall and membranes. Add tca (trichloroacetic acid) to the sand-cell mixture and. Proteins and nucleic acids are insoluble in tca and therefore will remain in suspension. Starch and glycogen are soluble in tca and therefore, will go into the solution of tca. Centrifugation: fractionation technique in which centrifugal force sediments suspended particle at the bottom of the tube. The sediment is known as a precipitate (or pellet) and the liquid remaining above in the supernatant. Transfer contents to 4 clean large glass test tubes. Nacl and will go into the solution (after centrifugation, Transfer to 4 clean plastic test tubes and centrifuge. Decant the four supernatants into a clean beaker.
