BIOL239 Lecture Notes - Hybridization Probe, Centromere, Prnp
Document Summary
Set 15: type ii ren (restriction endonuclease) The rest are cut with the same or different enzymes. What we"re staining with tends to stain double stranded things like dna but not always dna. The glowing bobs are (form hydrogen bonds like dna) trna molecules (linked to proteins and forms secondary structure) People use rnase which degrades rna so you don"t see it. It doesn"t make a difference: restriction maps. Show the order of different size fragments within a clone. Consist of linear (or circular) diagrams of the sites along a dna segment at which one or another restriction enzyme cleaves the molecule: molecular cloning (dna fragments) (dna cloning) Copies will be made through replication (e. coli treats it like it is its own). These are therefore known as dna clones: types of vectors. Each has an origin of replication, several unique sites for restriction enzymes and a gene referred to as a selectable marker.