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Biology 3326F/G Lecture Notes - Sodium Chloride, Lysis Buffer, Apoptosome

139 views21 pages
bluefox53
24 Sep 2014
School
Western University
Department
Biology
Course
Biology 3326F/G
Professor
Alexander Timoshenko

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Document Summary

We will discuss the logistics of the course, expectations, how to find a supervisor/project, answer your questions and hear experiences from current students enrolled in the course. Poster project: goal is to study the effects of heat shock on various aspects of bhk-21 and cho- The cytoskeleton state: to develop hands-on skills required for: Bioimaging of the cytoskeleton using regular and fluorescence microscopy. Use the paper by fulda et al 2010 as a general reference for cellular stress responses. Under normal conditions, heat shock transcription factor (hsf1) is held in the cytoplasm in its inactive state through direct protein sequestration by hsp90. When the cell is exposed to stressful conditions, there is accumulation of unfolded proteins which compete with hsf1 for hsp90 binding leading to hsf1 release from the complex. The hsf1 monomer rapidly trimerizes and translocates to the nucleus. Concurrent with multimerization, postranslational modification of hsf-1 is required for its trans-activating competence.

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Related Questions

biology

Module 1 - SLP

Cell Structure

Overview

For your Session Long Project, SLP, you will analyze currentscientific studies related to the important events such as theDeepwater Spill.

In Modules 1-5, you will work through the results of thisstudy:

Urakawa, H., Garcia, Juan C., Barreto, Patricia D., Molina,Gabriela A., Barreto, Jose C. (2012). A sensitive crude oilbioassay indicates that oil spills potentially induce a change ofmajor nitrifying prokaryotes from the Archaea to the Bacteria.Environmental Pollution. 164:42-45

For Module 1:

  1. First read this article from Scientific American:
    Clean Up the Deepwater Horizon Oil Spill
  2. Next, define in complete sentences using a paragraphformat:
  • What is the difference between a prokaryotic and a eukaryoticcell?
  • The following terms:

Archaea

Nitrifying bacteria

Ammonia-oxidizing bacteria

Gram-negative vs gram-positive bacteria

Hydrocarbon

Bioassay

Microtox bioassay

Crude oil

Biodegradation

  1. Finally read:

    Urakawa, H., Garcia, Juan C., Barreto, Patricia D., Molina,Gabriela A., Barreto, Jose C. (2012). A sensitive crude oilbioassay indicates that oil spills potentially induce a change ofmajor nitrifying prokaryotes from the Archaea to the Bacteria.Environmental Pollution. 164:42-45
  2. Which groups of bacteria were affected differently in thisstudy?

SLP Assignment Expectations

The Session Long Project consists of an integrative projectemphasizing the personalized application of each module's concepts.For Modules 1�5, students are required to engage in an originalintegrative project reflecting their comprehensive knowledge of andability to apply the course materials. Each component of the SLPwill be graded on a modular basis.

Your essay is considered a scholarly work. You will be providedwith many scholarly references to begin each assignment. For anyadditional research you are required to do to complete yourassignment, please use scholarly references such as a peer reviewedjournal article or a government sponsored or university sponsoredwebsite. As you read through your sources, take notes from yoursources and then write your paper in your own words, describingwhat you have learned from your research. Direct quotes should belimited and must be designated by quotation marks. Paraphrasedideas must give credit to the original author, for example (Murray,2014). Direct copying from �homework help� websites will notreceive credit.

When you write your essay, please organize your paper to includean introduction (overview of the assignment), body with subtitles(reflecting the requirements of the assignment), and a summary(develop connections between the required topics).

biology

Module 1 - SLP

Cell Structure

Overview

For your Session Long Project, SLP, you will analyze currentscientific studies related to the important events such as theDeepwater Spill.

In Modules 1-5, you will work through the results of thisstudy:

Urakawa, H., Garcia, Juan C., Barreto, Patricia D., Molina,Gabriela A., Barreto, Jose C. (2012). A sensitive crude oilbioassay indicates that oil spills potentially induce a change ofmajor nitrifying prokaryotes from the Archaea to the Bacteria.Environmental Pollution. 164:42-45

For Module 1:

  1. First read this article from Scientific American:
    Clean Up the Deepwater Horizon Oil Spill
  2. Next, define in complete sentences using a paragraphformat:
  • What is the difference between a prokaryotic and a eukaryoticcell?
  • The following terms:

Archaea

Nitrifying bacteria

Ammonia-oxidizing bacteria

Gram-negative vs gram-positive bacteria

Hydrocarbon

Bioassay

Microtox bioassay

Crude oil

Biodegradation

  1. Finally read:

    Urakawa, H., Garcia, Juan C., Barreto, Patricia D., Molina,Gabriela A., Barreto, Jose C. (2012). A sensitive crude oilbioassay indicates that oil spills potentially induce a change ofmajor nitrifying prokaryotes from the Archaea to the Bacteria.Environmental Pollution. 164:42-45
  2. Which groups of bacteria were affected differently in thisstudy?

SLP Assignment Expectations

The Session Long Project consists of an integrative projectemphasizing the personalized application of each module's concepts.For Modules 1�5, students are required to engage in an originalintegrative project reflecting their comprehensive knowledge of andability to apply the course materials. Each component of the SLPwill be graded on a modular basis.

Your essay is considered a scholarly work. You will be providedwith many scholarly references to begin each assignment. For anyadditional research you are required to do to complete yourassignment, please use scholarly references such as a peer reviewedjournal article or a government sponsored or university sponsoredwebsite. As you read through your sources, take notes from yoursources and then write your paper in your own words, describingwhat you have learned from your research. Direct quotes should belimited and must be designated by quotation marks. Paraphrasedideas must give credit to the original author, for example (Murray,2014). Direct copying from �homework help� websites will notreceive credit.

When you write your essay, please organize your paper to includean introduction (overview of the assignment), body with subtitles(reflecting the requirements of the assignment), and a summary(develop connections between the required topics).

Written Assignment: Biology and Technology in the Real World

Addresses course outcomes 1-4:

recognize and explain how the scientific method is used to solve problems

make observations and discriminate between scientific and pseudoscientific explanations

weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific method

use knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusions

1. Select one of the topics listed below.

2. Find at least three information sources related to the topic. You can find assistance with searching for articles at the UMUC Library Subject Guides at http://libguides.umuc.edu/science.

3. Write a 750-1500 word paper, excluding references and title page. You must read the information sources that you find and summarize the information in your own words, addressing each of the questions and expectations for your chosen topic. Extensive quotes from the article are discouraged. Use APA style for citing references, see https://www.umuc.edu/library/libhow/apa_tutorial.cfm

4. Post your assignment to the Assignment folder by the due date listed in the course schedule.

Topics (select one from the list below or from the expanded list of topics posted on November 16th in the announcements section)

a) Genetically modified organisms (GMOs). What is the purpose genetic engineering of crop plants and domestic animals? Briefly explain how GMOs are created. What foods in your supermarket contain GMOs? Are foods that contain GMOs safe for human consumption? What types of regulations exist for these foods? Clearly explain your reasoning for each answer.

b) Stem cells. Your friend had a spinal cord injury after a bad car accident. The medical team has decided that he is a good candidate for a clinical trial using stem cell therapy. Your friend has not had a biology course since high school, so you decide to write him a letter sharing your knowledge of stem cells. Include in your letter a description of the biology of stem cells and how these cells are unique from other cells. Contrast the different types of stem cells, including pros and cons of each. Explain how stems cells are can be used to treat diseases and injury, with special focus on spinal cord injuries. Conclude with your own opinion.

c) Fracking (hydraulic fracturing) and tar sands (oil sands). With society's dependence on nonrenewable fossil fuels, the oil & gas industry is turning to the use of hydraulic fracturing and tar (oil) sands to extract natural gas and oil respectively. A friend asks you "What's all this controversy in the news about fracking and tar sands?" Briefly explain to your friend how hydraulic fracturing and tar (oil) sands are used to obtain these fossil fuels. Then, in more detail, describe the environmental problems that may result from these processes and why they are controversial. Issues that may be addressed involves, but are not limited to, water, air and soil pollution, effects on human health, effects on other species and natural ecosystems. Finally, give your opinions on possible solutions to these environmental problems, with your reasoning backed by the references that you studied.