CSB452H1 : Summary of Lecture 1

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crimsonwombat705

24 Dec 2010

School

UTSG

Department

Cell and Systems Biology

Course

CSB452H1

Professor

Darrel Desveaux

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Document Summary

Pathogen associated molecular pattern can be flagellin, lipopolysaccharides of gram-negative bacteria, chitin, ergosterol, transglutaminase from fungi, or bacterial translation elongation factor ef-tu. Induces mapk cascade, transcription of pr genes and deposition of b1-3-glucn callose at infection site visualized by analine blue dye. Pamp cause plant to uptake h+, release k+ and increase ph causing alkalinization of media. apply bacterial protein extract separated by sds-page and see which band causes alkalinization. when flg22 is applied to plant before infiltration, bacterial growth decreased afterwards. Plants are then selfed to produce homozygous recessive mutant. wt seedling exposed to flg22 show stunted growth, so screen for insensitive mutant that grows normally with flg22. They do no accumulate callose and are more susceptible to. Pseudomonas syringae infection, growth and invasion into tissue using spray method. Infiltration method caused no difference showing fls2 is not involved in post invasion defence.

Related Questions

In a CaCl2 chemically competent bacterial transformation protocol, the role of the Ca2+ is to...

A. helps in shielding the negative charges present on the DNA and host cell membrane, thus lowering repulsion.

B. increases the bacterial cellâs ability to incorporate plasmid DNA.

C. act as a preserving agent as the prepared competent cells need to be stored at â 80oC.

D. allows the binding of plasmid DNA to Gram-negative bacteria lipopolysaccharide.

copperllama79

Q79 Which statement is FALSE in regards to the plasma membrane?

A Oxygen can easily diffuse across the plasma membrane.

B Glucose cannot easily diffuse across the plasma membrane.

C Potassium ions cannot easily diffuse across the plasma membrane

D Protons can easily diffuse across the plasma membrane.

E Water can easily diffuse across the plasma membrane.

Q80 Which of the following terms would best summarize the function of the general secretory pathway

A Translocase

B Transaminase

C Peptide Entry Facilitation

D Transacetylase

Q82 Which compound binds the outer membrane of Gram-negative bacteria to the thin peptidoglycan layer?

A porin

B lipoproteins

C polysaccharide

D bactoprenol

E lipoteichoic acid

Q83 Which of these gene products appears to confer a survival advantage on bacteria living in a competitive environment?

A Ton B

B Flagellin

C ExhB

D Ton B dependant receptors

E ExbD

Q84 What would be the gram reaction of Mycoplasma?

A Gram+ve bacteria

B Gram variable bacteria

C Gram -ve bacteria

Q85 A major difference between Gram positive and Gram negative cell walls is the presence of:

A Teichoic acids in Gram negative walls

B periplasm in Gram positive walls

C peptidoglycan in Gram positive walls.

D lipopolysachharides in Gram Negative walls

Considering the pathology of a cytokine storm, select possible strategies that would be likely to diminish the harmful effects of superantigen toxins. Select all of the strategies that would be likely to block or reduce the harmful effects of superantigen toxins.

Neutralizing circulating cytokines

Blocking the separation of the A and B components of the exotoxin

Blocking secretion of proteins by bacterial cells

Blocking molecular determinants on superantigens that interacts with T cells

Blocking the synthesis of lipopolysaccharide in gram-negative bacteria

Blocking host cell receptor-mediated endocytosis

Blocking the release of cytokines from T cells

cerisegerbil393

CSB452H1 : Summary of Lecture 1

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