CSB452H1 Lecture 4: Lecture 4 Summary
24 Dec 2010
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agrobacterium is gram-negative bacteria related to rhizobium. Agrobacterium enter via wound, live intercellularly and induce auxin, cytokinin mediated tumour. T4ss transports both protein and dna, use virb11 atp hydrolysis, vird4 bind t-dna, virb2 is pilus. Flg22 of agrobacterium is divergent, not a pamp, but ef-tu is a pamp. mutant receptor of ef-tu (efr-1) plant show more b-gluc transfer into plant genome by agrobacterium and more bacteria growth. Plant transformation needs disarming ti by removing tumour genes on t-dna and adding your gene to a much smaller size plasmid flanked by lb and rb. vir gene in agrobacterium act in trans to excise t-dna. Only lb and rb is required to flank your gene. Also need selectable marker and plant promoter before your gene. only some plant cell is transformed: transfer plant tissue onto selective medium to screen out. contain most tumour genes, agrobacterium lacking ti do not form galls.
