CSB428H1 Study Guide - Immunoprecipitation, Epistasis, Cantharidin

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crimsonwombat705

24 Dec 2010

School

UTSG

Department

Cell and Systems Biology

Course

CSB428H1

Professor

U.Tepass

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Document Summary

baz phosphorylation site bind pdz of sdt to recruit sdt to pm. Phosphorylation of baz causes it to dissociate from sdt. Non-phosphorylatable baz blocks dissociation causing mutation similar to crb and sdt. crb cytoplasmic domain binds pdz domain of sdt and sdt recruit patj and lin-7 to apical complex. dlg/lgl/scrib localize to lateral pm and antagonize crb-sdt complex, restricting apical expansion. wt baz-gfp and bazs980e-gfp shows the same localization basal to crb-sdt. Phosphorylated bazs980-p was concentrated in most apical part, sometimes with/without crb-sdt. wt baz-gfp and bazs980e-gfp but not bazs980a-gfp rescued maternal and zygotic baz lethality. Overexpression of bazs980a-gfp is similar to crb and sdt. overexpression of bazs980a-gfp caused formation of aggregate containing de-cad, arm, -cat, bazs980a-gfp overexpression caused cells to round up and die of apoptosis. However, those lacking pm targeting signal was not dominant, showing bazs980a-gfp must be at pm to be dominant.

Related Questions

Cyclin B1, a key cell cycle regulatory protein in vertebrates, is mostly cytosolic before mitosis. Early in mitosis, however, the protein is phosphorylated by certain protein kinases and consequently accumulates in the nucleus. How can phosphorylation bring about nuclear accumulation of this protein?

A. Phosphorylation within the nuclear localization signal inhibits the function of the signal.

B. Phosphorylation within the nuclear export signal enhances the function of the signal.

C. Phosphorylation within the nuclear export signal interferes with the function of the signal.

D. Phosphorylation elsewhere on the protein enhances binding to cytosolic proteins

fuchsiasea-lion54

Which of the following accurately describe difference(s) between oxidative phosphorylation and substrate-level phosphorylation during aerobic respiration in eukaryotes?

	a.	Oxidative phosphorylation relies on energy initially derived from redox reactions to power ATP synthase, and substrate-level phosphorylation relies on enzymatic reactions to phosphorylate ADP.
	b.	Oxidative phosphorylation takes place in the inner mitochondrial membrane and substrate-level phosphorylation only occurs in the cytosol.
	c.	Oxidative phosphorylation uses oxygen as a substrate and substrate-level phosphorylation uses phosphate.
	d.	Oxidative phosphorylation takes place during glycolysis and the electron transport chain and substrate-level phosphorylation occurs during the citric acid cycle and chemiosmosis.
	e.	Oxidative phosphorylation produces less ATP than substrate-level phosphorylation.

Despite their different names, conceptually oxidative phosphorylation and substrate level phosphorylation are similar. Which of the following are similarities between the two processes?

a. An energy source is oxidized.

b. An electron acceptor is reduced.

c. ADP is phosphorylated.

d. TWO of the above.

e. ALL of the above.

CSB428H1 Study Guide - Immunoprecipitation, Epistasis, Cantharidin

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