Biology 3594A : Genetics Tutorial II Notes
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22 Dec 2011
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A graduate student wants to compare the genome-wide gene expression patterns in normal cells and breast cancer cells. His advisor told him to purify mRNAs from both samples, convert mRNAs to cDNAs, use parallel-sequencing method (e.g. the pyro-sequencing talked in the class) to sequence the resulting cDNA, and then count the copy numbers of cDNA in each sample. This pipeline of experiments is termed as
cDNA sequencing | ||
RNA sequencing | ||
microarray | ||
hybridization |