Biology 3594A Lecture : RMC Notes
Document Summary
A random mutation capture assay to detect genomic point mutations in. Use a labelled flow chart and written description. In the paper, there are two pages about the assay - one in a written description and one in a picture description. What is the difference between mf and mf: mf - phenotype and is mutant frequency - what you see in a point in time. Blue stains you see or plaques you gather. It measure mutation burden phenotypically. mf - mutation frequency. Going to get rid of repeats that are clones of the original mutation. Will be a smaller number because of this. Just looking a the number of mutation events that caused the mutation burden. What is the effective mutation target size for cii assay and rmc assays: for the rmc assay, the effective mutation target assay is four, for cii it is 249.