BCH210H1 Lecture : lec30 soon-yi part 4 ETC and ATP counting

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10 Jan 2012

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Case study: soon-yi part iv: how atp is made in mitochondria: electron transport: the complexes, energy in electron flow and p/o ratios, atp accounting glycolysis in krebs, mitchell s chemiosmotic theory and atp generation mechanism. Soon-yi part 4: halfway at the waterworks: how atp is made in mitochondria. As soon-yi crests the hill at the east end of the beach she turns back to the boardwalk for the second half of her run as she heads west towards woodbine. Important question: how do mitochondria make atp via the energy in nadh, fadh2. Soon-yi now is an efficient atp generating machine. This is brought about by the electron transport chain in the inner mitochondria membrane. Electrons are transferred down a series of carriers of lower energy: energy is released as electrons go down carriers, adp is phosphorylated to make atp an water.

Related Questions

Define oxidative phosphorylation.

Describe the compartments of the mitochondria and where the electron transport chain

proteins are.

Relate the concepts of reduction potential and free energy (be able to calculate ÎEÂ°â and ÎGÂ°â

using ÎEÂ°â) and how this is coupled to transport of H+ out of the matrix.

Describe the role of the proton gradient in the function of ATP synthase (chemiosmotic theory)

Understand how the citric acid cycle, electron transport chain, and ATP synthase are linked

1. Oxidative Phosphorylation and the electron transport chain (ETC)

a. How are H⁺ being pumped across the inner membrane?

b. Why is the proton gradient important in oxidative phosphorylation?

c. What are the mobile carriers (ubiquinone and cytochrome c) in the ETC doing?

d. What is meant that with the subsequent proteins in the ETC, each has a greater redox potential?

e. From where are the H⁺ ions coming from which are being pumped across the inner mitochondrial eemembrane?

f. Where would the enzymes for citric acid cycle be found?

g. Where are the protein complexes for the electron transport chain found?

I have answered 1.) 92 2.)30 3.)? 4.) 12.5 5.) ? Any help wouldbe awesome

Glycolysis, ETC, and Krebs cycle biochemistry product questions:(This is biochemistry)

1. If you created an artificial mitochondria and injected 1molecule of pyruvate inside it, how many protons would accumulatein the intermembrane space of the mitochondria (starting from 1molecule of pyruvate take it all the way to the end of the electrontransport chain). 46 92 104 32

2. If you have created an artificial mitochondria and areinjecting ONE molecule of Pyruvate inside it, how many molecules ofATP can be theoretically generated starting from 1 molecule ofPyruvate ( start from pyruvate and complete all the reactions tofinish oxidative phosphorylation). Remember you also need to takeinto account any ATP generated via substrate Level phosphorylation.11.5 12.5 32 30

3.) If you have generated an artificial mitochondria and haveinjected 1 molecule of Acetyl CoA inside it, how many protons cantheoretically accumulate in the intermembrane space of themitochondria at the end of electron transport chain? 46 32 4636

4.) If you have generated an artificial mitochondria and haveinjected 1 molecule of Acetyl CoA inside it, how many ATP moleculescan theoretically accumulate in the intermembrane space of themitochondria at the end of electron transport chain and oxidativephosphorylation? ( Remember to take into accound ATP moleculegenerated via Substrate Level Phosphorylation) 12.5 9 10 32

5.) You have an artificial mitochondria, and you inject into it10 molecules of a compound X that can donate a pair of electrons tocomplex III of the mitochondria, how many protons can theoreticallyaccumulate in the intermembrane space of the mitochondria ? 60 100104 112

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BCH210H1 Lecture : lec30 soon-yi part 4 ETC and ATP counting

BCH210H1 Full Course Notes

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