MGY299Y1 : Lecture notes + tips for report
23 Jan 2012
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January 23 2012 y * in table and indicate below table that i interpreted this as Api biochemical tests way to identify unknown. Another way to identify by 16s rrna sequencing. Fairly reliable - long sequence 99% match: match between sequence of interest and database sequence. Same genus are the primers able to distinguish the species. 8fpl forward primer: 800 base pair sequence interested in. If sequences really close cannot use those primers to distinguish those organisms. Maximize how much you get out of your data. Within two hours, cultures did clear lysis know virus is growing this is a lytic infection. 30 to 300 300 issue of being able to count accurately; 25-30 is fine, but issue of statistical reliability towards lower end. If all within counting range, use all regardless of dilution factor being different: average. If variation (error bars, aka) is high data less reliable. If three or more calculating standard deviation.
