BIO130H1 Chapter : Text Notes BIO130 Lab 2

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9 Feb 2012
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BIO130H1 Full Course Notes
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Analyzing and manipulating data cleavage of dna at specific sites by restriction nucleases allows for isolation and manipulation of individual genes. Dna ligation design and construct dna molecules not in nature. Dna cloning use of cloning vectors or polymerase chain reaction (portion of. Monitor levels of mrna produced by every gene in a cell with nucleic acid arrays tens of thousands of hybridization reactions take place simultaneously. Restriction nucleases gene not a discrete entity small region of much longer dna molecule. Dna molecules produced through splicing two dna fragments together recombinant dna molecules. Dna molecules before electrophoresis often 32p because it can be incorporated into dna phosphates and emits particle easily detected by autoradiography. Dna cloning can mean: act of making identical copies of dna molecule isolation of a particular stretch of dna from the rest of a cell"s dna greatly facilitated by making many identical copies of the stretch of.

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