FREN 2500 Final: La Transposition

LINE1 element is a transposable element found in human genome.

Some student observed that copy element of the LINE1 element was increased in old population by genome sequencing analysis.

She wants to know whether aging indeed increases transposition of transposable element.

To measure transposition, she wants to make an assay to measure transposition first.

Based on LINE1 transposition uses mRNA transcription for transposition, design an assay using Green Fluorescence Protein gene.

What role does transposition serve in the phenomenon of antibiotic resistance in bacteria? Explain the actions of all of the enzymatic mechanisms through which DNA transposition is achieved. (4 marks)

2. What role does transposition serve in the phenomenon of antibiotic resistance in bacteria? Explain the actions of all of the enzymatic mechanisms through which DNA transposition is achieved. (4 marks)