BIOC58H3 Lecture : Tutorial 2

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orangeherring876

14 Feb 2011

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It is very important that you do the readings before you come to class. I will be putting you into groups so that you can answer some questions. I know it is hard to have discussions with a class this large. After doing your readings i would like you to come up with a question and answer. In future sessions if there are not a lot of volunteers i will pick a name from the list and you will be expected to have your question to give to the class and also have your answer. I will hand out papers and you will form your groups. Each group has to have a spokesperson that i will call on when needed. When you are finished send someone from your group up to tell me when you are done. If people have questions it is easiest to send someone from your group to ask me.

Related Questions

This is a post-lab question for my genetics class:

How can you prove that the substance you have extracted is DNA? Brainstorm with your group members (not possible as this is a solo post lab [until I can find a study group]) and outline an experiments that could be conducted to test your hypothesis. Present your group's idea in a teacher-mediated classroom forum (not possible, this will be typed and submitted) and hold a discussion with classmates about whether or not your chosen method would work.

The lab we preformed the Strawberry DNA Extraction, I think even little kids at science fairs have done this one!

The âhintâ given was to think about the chemical nature of DNA.

I understand that DNA is made of deoxyribonucleic acid. Deoxyribose being a specific type of sugar. There is also a phosphate and more importantly (or at least I think) is the nitrogenous base.

For Post 1 you should:

Find a relevant article or other information source (one that has not already been cited in your group Discussion)

Summarize a key point from this article/source.

Include a statement that specifically describes how this point is relevant to your question.

Pose one question that arises from reading the selected article that will stimulate discussion within your team.

Cite your reference in proper format (Links to an external site.) (AMA Style).

Remember that your ultimate goal is to review the Kenmore project and to determine whether there any significant concerns about feeding cattle:

a recombinant organism (that harbors an antibiotic resistance gene)

antibiotics (needed to maintain the MMO expression vector in coli)

And also remember that your Discussion Team is concerned specifically with the question:

FOR CONSUMERS OF MILK AND MEAT, what are the benefits/risks associated with the addition of antibiotics and genetically modified E. coli to cattle feed?

Lab Topic 1: The Process of Science

Introduction

1. Which of the following questions would you consider appropriate scientific questions that can be tested using the scientific method? For the statements that can be tested using the scientific method, explain how your experiment would be set up. Explain the different steps in the scientific method as they relate to this statement.

a. Does the pH of the soil affect the color of hydrangea flowers?

b. What properties of the soil influence the color of hydrangea flowers?

c. Do hydrangeas look more beautiful when grown in the right soil?

d. Was The Day After Tomorrow a better movie than Terminator?

e. Does grass require fertilizer in order to grow?

2. Read this hypothesis and answer the questions below:

Plants kept closer to windows grow larger than plants kept several feet away from windows.

a. Indicate the dependent variable:

b. Indicate the independent variable:

c. List 3 other variables that must be kept constant in this experiment (the control variables).

d. Write a prediction based on the above hypothesis.

Exercise 1.1

Take a Deep Breath

Students will need to take a picture of themselves or their group walking up and down on the step or doing other exercise. Include a note with your name and date on an index card in the picture. Insert picture here:

1. Identify the following in the experimental design. You will need to refer to the protocol to answer this question.

a. Independent variable:

b. Dependent variable:

c. Experimental dataset:

d. Control dataset:

e. Hypothesis:

2. Fill in the data for each individual in your group for the Breath experiment.

	Length of time breath can be held (seconds)
Individual Tested	Resting	After 1 minutes of exercise	After 5 minutes of exercise




Average (take the average for each column)

3. Look at the averages for all individuals in your group. Was the hypothesis you stated in the previous question supported by the âaverage dataâ? Explain your answer!!!

4. Now look at the data for each individual separately. Was there data for anyone in your group that did not support the hypothesis? If yes, explain whythis could be.

5. How many replicates did your group have?________________ Why is it good to have more than one replicate?

Exercise 1.2

Letâs Graph

In this exercise, you will be graphing the data that you collected in exercise 1.1.

1. In exercise 1.1, you identified the independent and dependent variables. Which one goes on the X axis of the graph and which goes on the Y axis?

2. Will you make a line or a bar graph of your data? Explain why you chose this type of graph.

3. You will need to use excel to generate a graph of your data. On this graph, you will need to label the title, independent variable, dependent variable and possibly the legend. You will need to select an appropriate scale so the data is spread out on the graph.

*Consult with your instructor to see how he/she would like for you to submit your graph. You might work on it in class and submit it through D2L or you might submit it at a different time. If you are taking the online version of the class, you will need to use Excel or word to make the graph.

Exercise 1.3

You will need to get the data under exercise 1.3 in the protocol sheet to complete these sections.

1. What is the dependent variable?

2. What is the independent variable?

3. Which, if any, of these individuals has diabetes? ___A or B ___?

4. What data do you have to support your hypothesis?

5. Construct a graph showing the time after eating each group had the greatest blood glucose levels.

BIOC58H3 Lecture : Tutorial 2

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