MBG 1000 Lecture Notes - Dna Microarray, Punnett Square, Angiogenesis

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beigetiger812

26 Mar 2012

School

University of Guelph

Department

Molecular Biology and Genetics

Course

MBG 1000

Professor

Hank Davis

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Document Summary

Indicates genes transcribed: uses microarrays (gene chips, mrnas are collected and cdnas created, cdnas bind to grid fluorescent signals display presence, relative amounts, usually compare control and experimental conditions. Rt enzyme makes dna from rna labelled red labelled green. In this step use heat to separate cdna strands. Computer scans colour and displays information, computer program can then analyze data and see how strongly the gene is expressed. Green signal of sample a is stronger than sample b. Yellow- sample a and sample b were present at = levels. This table summarizes the results of the experiment we were looking at above. Knowledge of other genes that are affected may lead to new treatments to alleviate the clinical symptoms of a human disorder resulting from a mutation in the human gene. Chapter 20 genetic testing, genetic counselling, and gene therapy. Interprets tests and explains the scientific and medical information: suggests courses of action, helps to cope.

Related Questions

1. What is the basic structure of a DNA nucleotide? What is the basic structure of a molecule of DNA?

Include: How are the carbons on the deoxyribose numbered? What do these numbers have to do with the 5â and 3â designations on a DNA strand?

2. If the sequence of one strand of a DNA molecule is: 5â â TATGC â 3â

Write out the sequence of bases on the complementary strand and indicate which end is 5â vs 3â?

3. DNA is a very stable molecule. What characteristics of its structure make it stable?

4. If 20% of the nitrogenous bases in the DNA of a guinea pig cell is adenine, what percentage is cytosine? Explain your answer. Which experiment/scientist led to this understanding?

silverantelope843

#3. ___________ is a molecule that serve as a cell's genetic information - a set of instructions that control the activities of the cell.

#9. The nitrogenous base adenine is represented by the letter _______.

#20. If one strand of DNA has the sequence A G T G, then the complementary strand will have the sequence ______.

#27. A mutated form of the gene for ____________ causes the disorder sickle cell anemia.

#32. _______ is the process of using DNA as a blueprint for making RNA.

#33. Portions of the RNA that are cut out and not used to make proteins are called ______.

1)What enzyme is used to attach different colors of stained nucleotides to create a DNA molecule along the mRNA?

Select one:

a. reverse transcriptase

b. fluorescent primer

c. restriction enzyme

d. radioactive probe

The process in which two complementary strands of DNA from different sources pair with one another is called hybridization.

Select one:

True

False

3) Explain the significance of a red microarray spot revealed by the microarray scanner.

Select one:

a. this gene is not expressed in either type of cell

b. this gene is expressed in cancer cells

c. this gene is expressed in healthy cells and cancer cells

d. this gene is expressed in healthy cells

4)What is the function of primers in PCR?

Select one:

a. raw material for new DNA

b. attach new nucleotides to DNA strand

c. attach to either side of target DNA

d. denature, or separate, DNA

5)What is the function of DNA polymerase in PCR?

Select one:

a. raw material for new DNA

b. attaches to either side of target DNA

c. attaches new nucleotides to DNA strand

d. denatures, or separates, DNA

MBG 1000 Lecture Notes - Dna Microarray, Punnett Square, Angiogenesis

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