BIOL 200 Lecture Notes - Forensic Science, Audiogram, Oligonucleotide Synthesis

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Molecular genetic methodologies ii: coli and bacteriophage lambda were, and still are, key elements in molecular biology. Replicational, transcriptional and translational mechanisms are nearly identical to higher eukaryotic organisms, although the molecules used in these mechanisms are different. Purified components such as proteins from these sources have helped a great deal in biological procedures. Cloning, biologically speaking is the ability to reproduce identical organisms from one source. It was in the late 60s and early 70s that the first successful cloning experiment was carried out on an animal. As a frog embryo developed into blastomeres which have not yet been determined, cloning was carried out by splitting each of these blastomeres which then each developed into one frog. Cloning can also be used for different purposes; taking the nucleus of a differentiated call and placing it into an innucleated site will cause nuclear reprogramming during which the nucleus becomes reprogrammed to the needs of the site.

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