KIN 155 Study Guide - Differential Interference Contrast Microscopy, Scanning Electron Microscope, Two-Dimensional Gel Electrophoresis
Document Summary
Brightfield microscopy basic form inexpensive and easy for color and fixed specimen and not for living species. Phase-contrast microscopy phase plate good for living, unstained specimen. Differential -interference -contrast microscopy (dic) (nomarski) polarizer analyzer. Confocal microscopy to produce 3-d image from a collection of optic sections. Use a beam of electron to produce an image. Coating with a layer gold or a mixture of gold and palladium. Source for the best yield fetal or neonatal tissue. What to do with a uniform population of cells. Proteins usually have a net positive or negative charge that reflects the mixture of charged amino acids they contain. If an electric field is applied to a solution containing a protein molecules, the protein will migrate at a rate that depends onits net charge and on its size and shape. They are assembly of lipids and proteins held together by noncovalent interactions.
