BIOL 3155 Study Guide - Cyclin-Dependent Kinase 9, Viral Infectivity Factor, Attenuated Vaccine
Document Summary
Rna sequences, not protein but present in protein coding regions: crs causes viral mrnas that have them to be retained in the nucleus, so the 9kb and. 4kb class remain in the nucleus: not the 2kb class (2x spliced) Both crs"s are removed from 2kb class and b/c of that it will not remain in nucleus, instead it will be transported to cytosol where it will be translated; so tat rev and nef are translated. Tat transactivator of transcription 2nd early protein translated from 2kb: tat is a highly basic protein - +ve charge, so it binds to nucleic acids cuz they"re negatively charged. It binds to rna: localizes to the nucleus activates transcription. Rev goes into nucleus and mediates their export: rev binds to rre (rev response element, rna sequence) in vrnas and. Rna elements are placed strategically in terms of their function. In env orf (present in 4kb and 9kb: fig.
