BIOB10Y3 Lecture Notes - Rna Polymerase Ii, Transcription Preinitiation Complex, Tata Box

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27 Sep 2012
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50 different species of trna = transfer rnas. Or: wash out the radioactivity for 3 hours after the pulse = chase, then lyse cells, extract rna and look to see where the radioactivity ended up. =hnrnas processed into smaller rnas that are exported to the cytoplasm = mrnas. All eukaryotic mrna precursors are synthesized by rna polymerase ii: enzyme has many different subunits. Requires the activity of various general transcription factors (gtfs: called general b/c their binding is required for transcription to initiate at many different promoters. Polymerase ii recognizes promoter regions located 5" of the transcription unit: b/w -24 and -32 is a specific promoter sequence known as the tata box, polymerase ii and the transcription factors assemble here = preinitiation complex. Tata box is bound by a protein known as the tata-binding protein = tbp. Tbp binding is required for rna polymerase ii to initiate transcription at the right place.

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