Biology 2382B Lecture Notes - Two-Dimensional Gel Electrophoresis, Adult Stem Cell, Monoclonal Antibody

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Cell culture the technique used to grow cells or tissues outside the organisms under strictly controlled conditions trypsin and proteolytic enzymes - break the cell-cell and cell-matrix interactions adn then you can culture those cells. Two types of cells that we can culture: primary cell culture is something extracted from tissue, while a cell line is immortal. Adult stem cells: finger-like projections call crypts (where our intestinal stem-cell niche is) Mesenchymal cells are signaling the intestinal cells to either divide or differentiate. Self renewal of adult stem cells is maintained by their interaction with: tumor cells restricted potential stem cells progenitor cells stem cell niche blood cells a. b. c. d. e. Atomic force microscopy - makes a 3d image, very high resolution, very high magnification. Resolution (d): ability to distinguish between two very closely positioned objects. Upright and inverted microscopes inverted: objective is below the stage, while in upright the objective is above the stage.

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