Biology 2382B Lecture Notes - Two-Dimensional Gel Electrophoresis, Adult Stem Cell, Monoclonal Antibody
Document Summary
Cell culture the technique used to grow cells or tissues outside the organisms under strictly controlled conditions trypsin and proteolytic enzymes - break the cell-cell and cell-matrix interactions adn then you can culture those cells. Two types of cells that we can culture: primary cell culture is something extracted from tissue, while a cell line is immortal. Adult stem cells: finger-like projections call crypts (where our intestinal stem-cell niche is) Mesenchymal cells are signaling the intestinal cells to either divide or differentiate. Self renewal of adult stem cells is maintained by their interaction with: tumor cells restricted potential stem cells progenitor cells stem cell niche blood cells a. b. c. d. e. Atomic force microscopy - makes a 3d image, very high resolution, very high magnification. Resolution (d): ability to distinguish between two very closely positioned objects. Upright and inverted microscopes inverted: objective is below the stage, while in upright the objective is above the stage.