BIOL 205 Chapter Notes - Chapter 4: Restriction Fragment Length Polymorphism, Zygosity, Restriction Enzyme
22 Nov 2012
School
Department
Course
Professor
Document Summary
Molecular heterozygote: different base pair at particular position on one chromatid than the other. When this variance is not linked to a particular phenotype, its called silent variation. Loci of molecular heterozygosity= molecular markers; act as mile stones on the map. -> most commonly used are single nucleotide polymorphisms and simple sequence length polymorphisms. In polygenes (qtls)- useful in discovering polygenes that contribute to a phenotype showing continuous variation. Intergenic snps- no measurable phenotypic effect; usedful as milestones: rflps- restriction fragment length polymorphisms= snps at restriction enzyme target site where there are 2 rflp alleles, one has target one does not. Can be mapped using sothern blot: see diagram 4-15 pg 148. Combinations of snps can be used to deduce linkage aswell as individual snps. Haplotype: chromosomal segment defined by specific aray of snp allels that it carries. -> inhereited through generations as blocks; over time crossingover may breakup haplotypes= finite life span.
