OC1049341

Have to exploit something that is different about your protein compared to all other proteins. *binding sites and substrates what ligands they have. So

Run on fblc on continuous trace to detect proteins. By the intensity, you can tell which fraction has your protein. Put together gel and want to load c

Have to exploit something that is different about your protein compared to all other proteins. *binding sites and substrates what ligands they have. So

The process by which there is an inhibition of substrate binding to enzyme. In absence of interfering agents, it just proceeds. Complex reaction mechan

Biochemical characterization begins with overexpression and purification of a target protein. Sometimes recombinant proteins can be created on an indus

They induce the activation energy, which affects the kinetics of the rxn. If they were evolved to be perfect binders, they would be receptors and would

Specificity only wants to see 1 band (ignore everything else) Sensitivity get enough protein to detect (get small amount of protein) Large glycoprotein

Polyacrylamide gel electrophoresis and transfer to pvdf membrane. Run complex mix of proteins in gel matrix to separate proteins based on their propert

Within 1 ph unit of the pka unit because its equally effective at buffering with conj base and conj acid. Little conj base to absorve the protons, mean

Brain: hypothalamus=homestasis balance in body ex: blood sugar, lateral region= hunger ; damage= stop eating, ventromedial region= depresses hunger ; d

Are there gender differences in love: francesca cansion- men and women have different styles, Expressive- talking and sinning about love, expressing fe

Way in which we record past events and knowledge: acquisition, storage, retrieval. Older ones that have weight is info processing approach: divides mem

Our capacity for storing long-term memories is essentially limitless. Brain is not like an attic that has a limit to storage. Thus despite brain"s vast

Which brain area responds to stress hormones by helping to create stronger memories: amygdala. This neural basis for learning and memory, found at the

Cross- cultural psychology and the study of sleep. Family that practices bed sharing: co-sleeping family bed , tiny baby is in the same bed. Co-sleepin

Most cultures have less insomnia than we do. Most likely to occur in women or men, but it is misleading because women usually seek help more than men d

Ex: child who sees sister burn fingers on hot stove learns not to touch it. Modeling: the process by which we observe and imitate specific behavior. Ex

What happens if after conditioning, cs occurred repeatedly without us (bell sounded but no food appeared, would their still be salivation?) Extinction:

Animal"s capacity on conditioning is constrained by its biology. To test: garcia exposed rats to particular taste, sight or sound (cs) and later to rad

If you reinforce every time, you use up all the treats. And out in the world, and if he always gets a treat for sitting and he doesn"t, he will be upse

Are dreams expression of unconscious wishes? (freud: latent, manifest. Are dreams the result of brain activity: activation-synthesis theory. Thought th