SLE234- Midterm Exam Guide - Comprehensive Notes for the exam ( 47 pages long!)

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Seminar 1 (15th april) ln an early experiment testing the hypothesis of spontaneous generation, francesco redi placed raw meat in beakers, covered some of the beakers with a fine cloth, and left the remaining beakers open. Maggots appeared in the open beakers but not the covered beakers. However, the meat in the covered beakers eventually did spoil. Suggest three possible sources of the organisms that spoiled the meat. Analysis of the dead cattle revealed tissue degradation in the brain (accounting for the erratic behaviour of diseased animals), but no foreign prokaryotic or non-cow eukaryotic dna or rna was ever dete(cid:272)ted i(cid:374) (cid:272)attle (cid:449)ith (cid:858)(cid:373)ad (cid:272)o(cid:449) disease(cid:859). Noital p(cid:396)o(cid:272)lai(cid:373)ed that (cid:858)(cid:373)ad (cid:272)o(cid:449) disease(cid:859) o(cid:271)eys ko(cid:272)h(cid:859)s postulates. Lack of nucleic aid indicated no organisms is present. Boiled beef broth is placed in two sterile flasks, flask a and flask b. Air treated with gamma radiation (dose sufficient to kill bacteria) is circulated through flask b.

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