BIOL2200 Study Guide - Midterm Guide: Integral Membrane Protein, Peripheral Membrane Protein, Choline
MEMBRANES
SAMPLE MCQ
1. WHICH STATEMENT ABOUT MEMBRANE PROTEINS IS INCORRECT?????
A. Integral membrane proteins can be released from the membrane with organic
solvents.
B. Alpha-helical structure is common in integral membrane proteins.
C. Integral membrane proteins use hydrophobic amino acids to interact with the fatty
acid chains found in lipids.
D. GPCRs are a large family of integral membrane proteins.
E.Integral membrane proteins contain no hydrophilic surfaces.
1. WHICH ONE OF THE FOLLOWING IS NOT A TYPE OF LIPID AGGREGATE STRUCTURE
A. Vesicle
B. Choline
C. Micelle
D. Bilayer
E.Liposome
SAMPLE SHORT ANSWER QUESTION
1. You have found two proteins in the water-insoluble fraction of the cell. They cannot
be solubilised by standard biological buffer at neutral pH. Protein 1, but not protein 2, could be
solubilised after adding a calcium-chelating agent. Protein 2 could only be solubilised after
adding 8M urea.
What do these experiments tell you about the membrane association of these two proteins? Is
any of them likely to be an integral membrane protein? Is any of them likely to be an
peripheral membrane protein?
What sort of interactions are proteins 1 and 2 likely to form with the membrane?
oHydrophobic interactions
A. Protein 1 likely forms ionic interactions with the membrane, mediated by calcium
ions. Protein 2 likely forms hydrophobic interactions with the lipid tails in the membrane.
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Document Summary
Integral membrane proteins can be released from the membrane with organic. Alpha-helical structure is common in integral membrane proteins. Gpcrs are a large family of integral membrane proteins. Integral membrane proteins use hydrophobic amino acids to interact with the fatty. Which one of the following is not a type of lipid aggregate structure. You have found two proteins in the water-insoluble fraction of the cell. They cannot be solubilised by standard biological buffer at neutral ph. Protein 1, but not protein 2, could be solubilised after adding a calcium-chelating agent. Protein 2 could only be solubilised after adding 8m urea. What sort of interactions are proteins 1 and 2 likely to form with the membrane: hydrophobic interactions. Protein 2 likely forms hydrophobic interactions with the lipid tails in the membrane.
