IMED1002 Study Guide - Final Guide: Polysome, Peptide Bond, Release Factor
RNA PROCESSING
______, ______ ad a log 3’ ed are all transcribed into ________. Getting to the _______ mRNA
transcript requires processing which can be divided into three main stages: __________,
____________ and _________. Since this processing takes place while the RNA is being transcribed,
it is referred to as _____________. Many of the enzymes and factors for processing are bound to the
____ of RNA Polymerase.
Capping involves the addition of a ___________ cap to the ___ end of the transcript. This protects it
from attack by ____________ and helps in _________ and _______ from the nucleus. Unusually like
the rest of RNA, the cap is joined via a ______ phosphodiester bond via a __________ linkage.
Before polyadenylation occurs, the pre-mRNA is __________ about ______ nucleotides
downstream of the __________ sequence (_________). ________ Polymerase then adds a poly A
tail which is important for ______________, _______________ and _____________.
Finally we get processing where _______ are spliced out and _______ are spliced together. The
exon-intron jutios otai ______________ uleotides (___ at the 5’ ed ad ___ at the 3’ ed
of the intron). These are recognised by _______ which are part of the ____________ together with
other proteins. The three sequences required for splicing are the splice ___________ site, the splice
_______ site and the _____________ (___).
Describe the splicing reaction
How can a single gene be used to produce different mRNAs and different proteins?
TRANSLATION
True/false:
Translation occurs in the nucleus F
UGA encodes methionine which is always the first AA in the chain F
The RNA sequence is read in groups of three called codons T
There are 3 possible reading frames but only one is used T
There are 3 stop odos that do’t ode for a AA T
Why is the code unambiguous and degenerate?
Almost every AA is encoded by more than 1 codon thus it is called a degenerate. A given codon only
codes for one AA is therefore called unambiguous.
Describe the structure and role of tRNA as well as the role of amino-acyl tRNA synthetases
Folds into a cloverleaf structure via base pairing, where anticodon loop base pairs with the mRNA
odo. It’s role is to trasfer AA that athes the RNA’s odos.
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Document Summary
______, ______ a(cid:374)d a lo(cid:374)g 3" e(cid:374)d are all transcribed into ________. Getting to the _______ mrna transcript requires processing which can be divided into three main stages: __________, Since this processing takes place while the rna is being transcribed, it is referred to as _____________. Many of the enzymes and factors for processing are bound to the. Capping involves the addition of a ___________ cap to the ___ end of the transcript. This protects it from attack by ____________ and helps in _________ and _______ from the nucleus. Unusually like the rest of rna, the cap is joined via a ______ phosphodiester bond via a __________ linkage. Before polyadenylation occurs, the pre-mrna is __________ about ______ nucleotides downstream of the __________ sequence (_________). ________ polymerase then adds a poly a tail which is important for ______________, _______________ and _____________. Finally we get processing where _______ are spliced out and _______ are spliced together.
