IMED1003 Study Guide - Final Guide: Oxaloacetic Acid, Mitochondrion, Glycogen
IMED1003: Final Revision Notes 1
LECTURE 7: TCA CYCLE - see full notes for more details
PDH Complex (3 enzymes):!
Final Reaction:!
Pyruvate + CoA + NAD+ ----(PDH)---> CO2 + NADH + Acetyl CoA
Regulation of PDH:!
IN EXAM: Important to know!
-Phosphatase ACTIVATES PDH!
-Kinase INHIBITS PDH!
TCA produces:!
3 NADH!
1 FADH2!
1 ATP!
2CO2!
Memorize regulatory enzymes:!
1. Citrate synthase!
2. Aconitase!
3. Isocitrate dehydrogenase!
4. Alpha-Ketoglutarate dehydrogenase complex!
5. Succinate dehydrogenase (IN THE MITOCHONDRIA MEMBRANE)!
6. Succinyl CoA synthetase !
7. Malate dehydrogenase!
8. Fumerase!
-** enzymes: mostly in matrix except 1 in mitochondria membrane!!!!!
Enzyme
Coenzyme
Pyruvate decarboxylase
Thiamine pyrophosphate (TPP)
Dihidrolipoyl transacetylase
Lipoic acid (acyl lipollysine) AND CoA
Dihidrolipoyl dehydrogenase
FAD AND NAD+
Inhibit PDH
Activate PDH
NADH
Pyruvate
Acetyl CoA
ADP
ATP
Document Summary
Lecture 7: tca cycle - see full notes for more details. Pyruvate + coa + nad+ ----(pdh)---> co2 + nadh + acetyl coa. Isocitrate dehydrogenase: alpha-ketoglutarate dehydrogenase complex, succinate dehydrogenase (in the mitochondria membrane, succinyl coa synthetase, malate dehydrogenase, fumerase. ** enzymes: mostly in matrix except 1 in mitochondria membrane!!! O2 is needed to make atp from nadh/fadh2. Acetyl coa = 2 c acetyl group. Entry of pyruvate occurs before the tca cycle. Oxidation of pyruvate to acetyl-coa & nadh. Acetyl-coa combines with 4c oxaloacetate = coa is released. 6 carbons from 2 pyruvates oxidised -> co2. 2. acetyl coa -> tca -> 3nadh, 1fadh2, 1gtp, 2co2 (per pyruvate) Also subject to covalent modi cation by pdh kinase & pdh phosphatase. They are the main products of glycolysis & tca cycle. They are electron carriers that produce atp. Electrons transferred from nadh + fahd2 to o2 = drives atp formation.
