Microbiology (please explain answers to first question!!):
1) The enzyme Beta-galatosidase can carry out the reaction ONP-galactose-->ONP+galactose. ONP is yellow. In the reactions you will do ONPG is present in large excess. If 0.1ml of a bacterial extract has enough enzyme to produce enough ONP to give an OD of 0.2 after an enzyme assay time of 10 min, then
a. What will the OD be after an enzyme assay time of 20 min?
b. What will the OD be after 10 min if I add twice as much extract?
c. What will the OD be after 10 min if I add three times as much extract?
d. What will the OD be if I take the extract from cells grown with glucose rather than lactose
2) What would the difference be between measuring units of enzyme/OD of cells and total units of enzyme/ml with a culture growing on lactose alone?
What will happen to each measurement if the number of cells doubles but the amount of enzyme per cell is constant?