BIOL 3P43 Study Guide - Final Guide: Corynebacterium Glutamicum, Flavor, Trp Operon
23 Apr 2019
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Document Summary
3 processes for manufacture of aa -> 1) extraction of aa from protein hydrolysates 2) chemical synthesis 3) microbiological production. 3 approaches to microbiological production -> 1) direct fermentation of aa 2) bioconversion of inexpensive intermediates (glycine -> serine) 3) immobilized enzymes. In nature microbes rarely produce aa in excess b/c of feedback inhibition & regulation (except for glutamate production) Allosteric enzyme means that there are two sites on the enzyme an effector can bind to this and can turn off enzyme. Proline can bind to allosteric site & turn it off if there is too much proline being produced then the enzyme is turned off. 2 scenarios: 1) mutants for proline overproduction- at the allosteric site microorganism will spit" it into the broth. 2) mutants for glutamate overproduction at the active site- will spit it out into broth. Arginine regulation repressor protein binds to the repressor site (down stream to the rna polymerase).
