BIOL 367 Study Guide - Hindiii, Southern Blot, Bamhi
30 Nov 2014
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A linear dna molecule is labelled at both ends with p32 and digested with restriction enzymes. Hindiii produces fragments of sizes 2. 9, 4. 5, 6. 2, 7. 4 and 8. 0 kb. An autoradiogram developed from a southern blot of this digest shows radioactivity associated with the 6. 2 and 8. 0 kb fragments. When sst1 cleaves the same fragment it generates three bands of 6. 0, 10. 1 and 12. 9 kb with the 6. 0 and 10. 1 being radioactive. When the same molecule is digested with both. Hindiii and sst1, fragments of sizes 1. 0, 2. 0, 2. 9, 3. 5, 6. 0, 6. 2 and 7. 4 kb are produced. Draw a restriction enzyme recognition site map showing the sst1 and. Hindiii sites: the following restriction fragments were obtained by a graduate student when she performed agarose gel electrophoresis analysis of a bacterial plasmid. Ecor1 (1. 0 kbp, 2. 2 kbp and 7. 1 kbp) Bamh1 and ecor1 (0. 4 kbp, 1. 0 kbp, 1. 8 kbp, 3. 2 kbp and 3. 9 kbp)
