BIOC 3400 Study Guide - Midterm Guide: Reverse Transcriptase, Nucleolus, Helicase
11 Nov 2015
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Study online at quizlet. com/_1qb45w bind distal regulatory sequences (e. g. , enhancers) A transcriptional activator is a protein (transcription factor) that increases gene transcription of a gene or set of genes. Most activators are dna-binding proteins that bind to enhancers or promoter-proximal elements. Are simply plasmids designed to propagate large dna fragments. Propagated at low copy number in e. coli. 100-300+ kb fragments can be maintained highly supercoiled dna complexed with basic proteins (not histones) tethered to the cell membrane. The filamentous material of chromosomes, comprised of dna and associated histone and non-histone proteins. a method of positional cloning used to find, isolate, and clone a particular allele in a gene library. Starting with the dna sequence of a single clone, probes can be used to screen a genomic library for additional clones that contain fragments overlapping with the original clone. Isolation of a particular nucleotide dna sequence from its genomic context.
