BIOC 3400 Study Guide - Final Guide: Protein Biosynthesis, Adar, Ribonucleoprotein
10 Dec 2015
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Unit 3 _ experiments, enzymes, & important questions. Some sirna unwind without being associated with risc. The antisense strand can bind to its complementary region of the corresponding mrna, serving as a primer for a rdrp which will generate new dsrna of the target gene. Rdrp generated dsrna is substrate for dicer, and more sirna is made. 5"-cap on mrna recognized, then ribosome scans mrna for first aug after cap. Start codon (aug) is located near the 5 -end of the mrna. 16s rrna of ssu recognizes specific sequence upstream of aug start codons. Recognition of the start codons is facilitated by the presence of a ribosome recognition motif (shine-dalgarno, or s-d, sequence). The purine-rich shine-dalgarno (s-d) sequence , just upstream (5 ) of the start (aug) codon, interacts, by complementary, antiparallel base pairing, with a pyrimidine-rich sequence (anti s-d sequence) very close to the 3"-end of the ssu (16s) rrna.
