JOUR 3440 Study Guide - Final Guide: Peptide, Prokaryote, Base Pair
24 Oct 2015
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Document Summary
An antibiotic blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to neomycin is conferred by the neo-r, neomycin resistance, gene. A drug kills cells containing a functional hsv-tk gene, but is not toxic to cells with only the cellular tk gene. An antiviral drug kills cells containing a functional hsv- tk gene, but is not toxic to cells with only the cellular tk gene. Cells containing this gene will be killed by ganciclovir. They are a class of engineered dna-binding proteins. They facilitate targeted editing of the genome by creating double-strand breaks in dna at user-specified locations. Fusion of nonspecific dna cleavage domain from the fokl restriction endonuclease with zinc-finger proteins. The binding specificity of the designed zinc-finger domain directs the zfn to a specific genomic site. Artificial restriction enzymes generated by fusing a tal effector. Dna binding domain to a dna cleavage domain.
